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| 4 | 488/532 | 555/20 | 561 | 572 | (in buffer): 2 (in antifade): 2 | microscopy, flow cytometry, fluorescence microplate reader |
Alexa Fluor 546 dye is a bright, orange-fluorescent dye that can be excited using the 488 nm or 532 nm laser lines. For stable signal generation in imaging and flow cytometry, Alexa Fluor 546 dye is pH-insensitive over a wide molar range.
Probes with high fluorescence quantum yield and high photostability allow detection of low-abundance biological structures with great sensitivity. Alexa Fluor 546 dye molecules can be attached to proteins at high molar ratios without significant self-quenching, enabling brighter conjugates and more sensitive detection.
We offer Alexa Fluor 546 dye, conjugated to a variety of antibodies, peptides, proteins, tracers, and amplification substrates optimized for cellular labeling and detection. In addition, reactive dye forms and protein labeling kits are available to allow you to generate your own antibody conjugates or probes.
Protein Labeling Reagents Selection Guide (NHS ester, maleimide, etc.) Search Alexa Fluor 546 secondary antibodies
Figure 1. The intermediate filaments in bovine pulmonary artery endothelial cells were visualized using an anti-desmin antibody, followed by Alexa Fluor 647 goat anti–mouse IgG antibody (purple). Endogenous biotin in the mitochondria was labeled with Alexa Fluor 546 streptavidin (red), and DNA in the cell was stained with DAPI (white).
Figure 2. Cultured axolotl sensory neurons stained with our anti-human neuronal protein HuC/HuD antibody (anti-Hu) and detected using the red-orange-fluorescent Alexa Fluor 546 goat anti-mouse IgG antibody. The image was contributed by Josh Gross and Linda Barlow, University of Denver, Denver, Colorado.