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5 | 514 | YFP | 512 | 529 | 5 (in antifade) | Microscopy |
Invitrogen Aluora dyes, including Aluora 514 dye, are a specialized organic dye technology specifically optimized for spatial biology and multiplex immunohistochemistry (IHC) applications. Aluora 514 dye is excited by the 514 nm laser and emits at 529 nm. This dye is bright and has exceptional photostability. To learn more about spatial imaging, visit our Spatial Biology Resource Center
We offer Aluora 514 spatial amplification reagents for use in immunohistochemistry.
Human FFPE small intestine was incubated with 1:1,000 of PCNA Monoclonal Antibody (PC10) (Cat. No. 13-3900) then labeled with the reagents in the Aluora 514 HRP-mouse Kit (Cat. No. A40001331) and a DAPI nuclear stain. The slides were mounted in ProLong Glass mountant. Images were captured with an Invitrogen EVOS M7000 instrument (see information about EVOS imaging systems).