dA(Bz) CEP
Designed for oligonucleotide synthesis for diagnostic and therapeutic development
Thermo Scientific DNA phosphoramidites are built to meet the varying needs of oligonucleotide manufacturers for the development of therapeutic, diagnostic, and research applications. We offer TheraPure, standard, and fast deprotect phosphoramidites.
- TheraPure phosphoramidites undergo additional quality control release testing and are compared with our standard phosphoramidites, helping to ensure that impurities and residual solvents are controlled to the stringent levels required by our customers for their oligo therapeutics.
- Standard phosphoramidites support the quality and reliability suitable for routine oligonucleotide synthesis.
- Fast deprotect phosphoramidites allow the removal of base protecting groups under mild or accelerated conditions without the need for special reagents.
Our DNA phosphoramidites are supported by:
- Expert technical support—over 40 years of partnership, customization, and scale-up experience
- Secure and consistent supply—5x capacity expansion in 2022
- Service excellence—48 hours or less response to orders, >98% delivery to customer at promised date†
- Flexible packaging—custom and bulk packaging available upon request
- Quality—ISO 9001:2015–certified quality management system that has been refined in partnership with our customers since 1995
†May not be applicable in all regions.
Quality manufacturing and regulatory documentation
Processes are monitored from material procurement through final QC and product release for batch-to-batch reproducibility. Purity of each phosphoramidite lot is assessed by HPLC-MS, GC, 31P NMR, as well as Karl Fischer oven titration. Our rigorously controlled manufacturing processes help minimize levels of reactive P(III) impurities which could be incorporated into the oligonucleotide during synthesis and result in the accumulation of by-products.
Available DNA phosphoramidites by synthesizer
| Applied Biosystems/AKTA Synthesizer | Expedite Synthesizer | MerMade Synthesizer | ||
|---|---|---|---|---|
| TheraPure phosphoramidites | Bz-dA | ✓ | ✓ | ✓ |
| Bz-dC | ✓ | ✓ | ✓ | |
| iBu-dG | ✓ | ✓ | ✓ | |
| dT | ✓ | ✓ | ✓ | |
| Standard phosphoramidites | Bz-dA | ✓ | ✓ | ✓ |
| Bz-dC | ✓ | ✓ | ✓ | |
| iBu-dG | ✓ | ✓ | ✓ | |
| dT | ✓ | ✓ | ✓ | |
| dU | ✓ | ✓ | ||
| dI | ✓ | |||
| Fast deprotection (mild) | PAC-dA | ✓ | ||
| Ac-dC | ✓ | ✓ | ✓ | |
| DMF-dG | ✓ | ✓ | ✓ | |
| iPrPAC-dG | ✓ |
For items not listed above, flexible/bulk packaging, or custom services, please contact us.
TheraPure DNA phosphoramidites
TheraPure phosphoramidites are manufactured with high purity and tight control of critical impurity level for excellent batch-to-batch reproducibility.
Click images to enlarge
dC(Bz) CEP
dG(iBu) CEP
dT CEP
| dA(Bz) CEP | dC(Bz) CEP | dG(iBu) CEP | dT CEP | |
|---|---|---|---|---|
| Cat. No. | 27-2030 | 27-2032 | 27-2034 | 27-2036 |
| Formula | C47H52N7O7P | C46H52N5O8P | C44H54N7O8P | C40H49N4O8P |
| Molecular weight | 857.95 g/mol | 833.93 g/mol | 839.92 g/mol | 744.82 g/mol |
| CAS # | 98796-53-3 | 102212-98-6 | 93183-15-4 | 98796-51-1 |
| HPLC purity | ≥99% | |||
| 31P NMR purity | ≥99% | |||
| Solution clarity | Free from undissolved particulate at 0.2 M solution in ACN | |||
| Appearance | White to pale yellow powder | |||
| Residual solvent | ≤3.0% total % by weight | |||
| Shipping/Storage condition | Ambient / –20°C | |||
| Water content | ≤0.3% | |||
| Material origin | Non-animal source (no TSE/BSE risk) | |||
Ordering information
Not finding the product you need? Please contact us or request custom services.
Standard DNA phosphoramidites
Traditional base protection methods are used to help ensure quality and reliability for routine oligonucleotide synthesis.
Click images to enlarge
dA(Bz) CEP
dC(Bz) CEP
dG(iBu) CEP
dT CEP
| dA(Bz) CEP | dC(Bz) CEP | dG(iBu) CEP | dT CEP | |
|---|---|---|---|---|
| Cat. No. | 27-1730 | 27-1732 | 27-1734 | 27-1736 |
| Formula | C47H52N7O7P | C46H52N5O8P | C44H54N7O8P | C40H49N4O8P |
| Molecular weight | 857.95 g/mol | 833.93 g/mol | 839.92 g/mol | 744.82 g/mol |
| CAS # | 98796-53-3 | 102212-98-6 | 93183-15-4 | 98796-51-1 |
| HPLC purity | ≥98.0% | |||
| 31P NMR purity | ≥98.0% | |||
| Water content | ≤0.3% | |||
| Shipping/Storage condition | Ambient / –20°C | |||
| Solution clarity | Free from undissolved particulate at 0.2 M solution in ACN | |||
| Material origin | Non-animal source (no TSE/BSE risk) | |||
Ordering information
Not finding the product you need? Please contact us or request custom services.
Fast deprotect DNA phosphoramidites
They are ideal for applications requiring mild deprotection to preserve the integrity of oligonucleotides containing sensitive dyes, labels, or other modifications that may be unstable with prolonged exposure to strong alkaline conditions. Fast deprotect phosphoramidites permit the removal of base protecting groups under mild or accelerated conditions without the need for special reagents.
Click images to enlarge
iPrPAC-dG CEP
PAC-dA CEP
Ac-dC CEP
DMF-dG CEP
| iPrPAC-dG CEP | PAC-dA CEP | Ac-dC CEP | DMF-dG CEP | IBU-dC CEP | |
|---|---|---|---|---|---|
| Cat. No. (TheraPure phosphoramidites) | N/A | N/A | 27-2027 | N/A | N/A |
| Cat. No. (standard phosphoramidites) | 27-1726 | 27-1723 | 29-1727 | 27-1737 | 27-1725 |
| Formula | C51H60N7O9P | C48H54N7O8P | C41H50N5O8P | C43H53N8O7P | C43H54N5O8P |
| Molecular weight | 946.05 g/mol | 887.96 g/mol | 771.8 g/mol | 824.9 g/mol | 839.92g/mol |
| CAS # | 150065-82-0 | 110543-74-3 | 154110-40-4 | 330628-04-1 | 110522-84-4 |
| HPLC purity | (TheraPure phosphoramidites) | ≥98.0% | ≥98.0% | ≥98% | (≥99%) | ≥98.0% | ≥98.0% |
| 31P NMR purity | (TheraPure phosphoramidites) | ≥98.0% | ≥98.0% | ≥98% | (≥99%) | ≥98.0% | ≥98.0% |
| Shipping/Storage condition | Ambient / –20°C | ||||
| Material origin | Non-animal source (no TSE/BSE risk) | ||||
Ordering information
Not finding the product you need? Please contact us or request custom services.
Resources
Technical note
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For Research Use Only. Not for use in diagnostic procedures.