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Alexa Fluorおよび Alexa Fluor Plus色素 |
Invitrogen Alexa FluorおよびAlexa Fluor Plus色素は、広く使用され信頼されている蛍光色素です。これらの色素はスペクトル的に多様で高性能の低分子フルオロフォアで、蛍光ベースのさまざまなアプリケーションに適合します。優れた性能に加えて、問題を解決するための経験豊富なテクニカルサポートチーム、80,000を超える公開済み文献、実験の計画を支援する豊富なアプリケーションおよび実験のヒントとプロトコルなどを活用できます。
Alexa FluorおよびAlexa Fluor Plus色素の概要
Invitrogen Alexa FluorおよびAlexa Fluor Plus色素の幅広いラインアップには、あらゆるフィルターに対応し、マルチプレックス検出のための多くのオプションがあります。この一連のフルオロフォアは、near-UV、可視光、および近赤外スペクトルにおよび、一般的な励起源や装置すべてと互換性があります。
| 色 | Alexa FluorおよびAlexa Fluor Plus色素 | 励起／蛍光 | 同等の色素 |
|---|---|---|---|
| Blue | Alexa Fluor 350 | 343／441 | AMCA, coumarin |
| Alexa Fluor 405 | 401／422 | ||
| Alexa Fluor Plus 405 | 404／455 | ||
| Green | Alexa Fluor 430 | 431／540 | |
| Alexa Fluor 488 | 499／520 | Cy2, FITC (fluorescein) | |
| Alexa Fluor Plus 488 | 493／518 | ||
| Alexa Fluor 514 | 518／543 | ||
| Yellow | Alexa Fluor 532 | 534／553 | |
| Orange | Alexa Fluor 546 | 561／572 | |
| Alexa Fluor 555 | 553／568 | Cy3, TRITC (rhodamine) | |
| Alexa Fluor Plus 555 | 558／572 | ||
| Alexa Fluor 561 | 556／569 | ||
| Red | Alexa Fluor 568 | 579／603 | Rhodamine red |
| Alexa Fluor 594 | 590／618 | Texas Red | |
| Alexa Fluor Plus 594 | 590／618 | ||
| Far Red | Alexa Fluor 633 | 631／650 | |
| Alexa Fluor 635 | 633／648 | ||
| Alexa Fluor 647 | 650／671 | Cy5 | |
| Alexa Fluor Plus 647 | 658／675 | ||
| Near-IR | Alexa Fluor 660 | 663／691 | |
| Alexa Fluor 680 | 681／704 | Cy5.5、IR680 | |
| Alexa Fluor Plus 680 | 687／704 | ||
| Alexa Fluor 700 | 696／719 | ||
| Alexa Fluor 750 | 752／776 | Cy7 | |
| Alexa Fluor 790 | 782／805 | ||
| Alexa Fluor Plus 800 | 789／794 |
Alexa FluorおよびAlexa Fluor Plus製品
Alexa FluorおよびAlexa Fluor Plusのリソース
Alexa Fluor色素の利点
抜群の明るさ
Alexa Fluor色素で作られたタンパク質コンジュゲートは、スペクトル的に類似した他の蛍光色素標識タンパク質と比較して高い蛍光を生成します。Alexa Fluor色素はさまざまな蛍光色から選択でき、他のほとんどの色素よりも光安定性に優れています。
Alexa Fluor色素分子は、大きな自己消光なしにタンパク質に結合できるため、イメージング、フローサイトメトリー、およびその他の蛍光ベースのアプリケーションに使用できる、より明るいコンジュゲートを提供します。
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Comparison of relative fluorescence of goat anti–mouse IgG antibody conjugates prepared from the Alexa Fluor 488 dye and fluorescein isothiocyanate (FITC).Conjugate fluorescence is measured by comparing the fluorescence quantum yield of the conjugated dye relative to that of a reference dye and multiplying by the dye:protein labeling ratio.
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Comparison of relative fluorescence of goat anti–rabbit IgG antibody conjugates of the Alexa Fluor 555 and Cy3 dyes at different dye:protein ratios in the conjugate.
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Flow cytometry comparison of the brightness of Alexa Fluor 647 goat anti–mouse IgG antibody (red, A21235) with Cy5 goat anti–mouse IgG antibody from Jackson ImmunoResearch Laboratories (green) and Amersham-Pharmacia Biotech (blue).Human blood was blocked with normal goat serum and incubated with an anti-CD3 mouse monoclonal antibody; cells were washed, resuspended and incubated with either an Alexa Fluor 647 or Cy5 goat anti–mouse IgG secondary antibody.Samples were analyzed on a flow cytometer equipped with a 633 nm He–Ne laser and a longpass emission filter (>650 nm).
光安定性の向上
光安定性はAlexa Fluor色素コンジュゲートの重要な特性であり、画像キャプチャに長い時間をかけることができます。Alexa Fluor色素および色素コンジュゲートでは、優れた輝度とスペクトル全体をカバーする幅広い色素に加えて、信頼できる光安定性も提供します。
Alexa Fluor色素分子の光安定性は、フルオレセインなどの従来の蛍光色素との比較で確認できます。
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Comparison of the photobleaching rates of the Alexa Fluor 488 and Alexa Fluor 546 dyes and the well-known fluorescein and Cy3 fluorophores.The cytoskeleton of bovine pulmonary artery endothelial cells (BPAEC) was labeled with (top series) Alexa Fluor 488 phalloidin and mouse monoclonal anti–a-tubulin antibody in combination with Alexa Fluor 546 goat anti–mouse IgG antibody or (bottom series) fluorescein phalloidin and the anti–a-tubulin antibody in combination with a commercially available Cy3 goat anti–mouse IgG antibody.The pseudocolored images were taken at 30-second intervals (0, 30, 90, and 210 seconds of exposure).The images were acquired with bandpass filter sets appropriate for fluorescein and rhodamine.
Reduced photobleaching, sustained signal.Bovine pulmonary artery endothelial cells were labeled with fluorescein phalloidin (A) or Alexa Fluor 488 phalloidin (B) which label filamentous actin.The cells were placed under constant illumination on the microscope.Images were acquired at one-second intervals for 30 seconds.Under these illumination conditions, fluorescein images are photobleached to about 20% of its initial value, while fluorescence of Alexa Fluor 488 phalloidin stayed at the initial value.
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Alexa Fluor Plus色素のさらなる利点
Alexa Fluor Plus色素による低発現量ターゲットの検出
従来のAlexa Fluor色素は、さまざまなアプリケーションでさまざまな発現レベルのタンパク質を検出するために使用できますが、Alexa Fluor Plus色素は、より高いシグナル対ノイズ比を提供し、交差反応性を最小限に抑えるように化学的に最適化されているため、希少サンプルや貴重なサンプル中の低発現量ターゲットを検出するのに役立ちます。
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Alexa Fluor Plus 405 conjugates allow for improved signal to noise when compared to traditional Alexa Fluor 405 conjugates.Traditional Alexa Fluor 405 secondary antibody (left) compared to Alexa Fluor Plus 405 secondary antibody (right, goat anti-rabbit IgG secondary antibody, Cat.No. A48254) in immunofluorescent analysis of β-III tubulin in fixed HeLa cells.Nuclei were stained with SYTOX Deep Red stain (Cat.No S11380).The images contain overlay of tubulin (cyan), and nuclei (magenta). Images were taken on an EVOS M7000 Imaging system (Cat.No. AMF7000) at 20x magnification.
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Alexa Fluor Plus 555 allows you to see greater detail compared to traditional Alexa Fluor 555 conjugates.Traditional Alexa Fluor 555 secondary antibody (left) compared to Alexa Fluor Plus 555 secondary antibody (right, goat anti-mouse IgG secondary antibody, Cat.No. A32727) in immunofluorescent analysis of β-III tubulin in E18 Sprague Dawley primary cortical neuronal cells.Nuclei were stained with Hoechst 33342 stain (Cat.No. H3570).The image contains overlay of neuronal tubulin (orange) and nuclei (blue).Images were taken on a confocal microscope at 40x magnification.
For Research Use Only. Not for use in diagnostic procedures.
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