Table 19.5 Response of fura-2 and indo-1 to some divalent cations other than Ca2+ and Mg2+.
| Ion | Indicator | Kd * | Spectroscopic Effect | Reference |
|---|---|---|---|---|
| Ba2+ | fura-2 | 1.4 × 10-6 M | Spectra similar to Ca2+ complex; no quenching |  |
| Cd2+ | fura-2 | 1.0 × 10-12 M | Spectra similar to Ca2+ complex; higher intensity | |
| Co2+ | fura-2 | 8.6 × 10-9 M | >99% Quenched relative to Ca2+ complex | |
| Mn2+ | fura-2 | 2.8 × 10-9 M | >99% Quenched relative to Ca2+ complex | |
| Pb2+ | fura-2 | 4.2 × 10-12 M | Spectra shifted relative to Ca2+ complex; no quenching | |
| Pb2+ | indo-1 | 3.5 × 10-11 M | Fluorescence quenched | |
| Sr2+ | fura-2 | 7.6 × 10-6 M | Spectra similar to Ca2+ complex; no quenching | |
| Zn2+ | fura-2 | 3.0 × 10-9 M | Spectra similar to Ca2+ complex; no quenching | |
| Zn2+ | indo-1 | 1.6 × 10-10 M | Spectra similar to Ca2+ complex; no quenching | |
| * Dissociation constant reported in cited literature reference. | ||||
For Research Use Only. Not for use in diagnostic procedures.