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Technical Reference Library
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The Molecular Probes Handbook
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Enzyme Substrates and Assays—Chapter 10
Enzyme Substrates and Assays—Chapter 10
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Chapter 10—Enzyme Substrates and Assays
Introduction to Enzyme Substrates and Their Reference Standards—Section 10.1
Detecting Glycosidases—Section 10.2
Detecting Enzymes That Metabolize Phosphates and Polyphosphates—Section 10.3
Detecting Peptidases and Proteases—Section 10.4
Substrates for Oxidases, Including Amplex Red Kits—Section 10.5
Substrates for Microsomal Dealkylases, Acetyltransferases, Luciferases and Other Enzymes—Section 10.6
Molecular Probes Handbook
Table of Contents
Fluorescence Fundamentals
Chapter 1—Fluorophores and Their Amine-Reactive Derivatives
Chapter 2—Thiol-Reactive Probes
Chapter 3—Click Chemistry and other Functional Group Modifications
Chapter 4—Biotin and Hapten Derivatives
Chapter 5—Crosslinking and Photoactivatable Reagents
Chapter 6—Ultrasensitive Detection Technology
Chapter 7—Antibodies, Avidins and Lectins
Chapter 8—Nucleic Acid Detection and Analysis
Chapter 9—Protein Detection and Analysis
Chapter 10—Enzyme Substrates and Assays
Chapter 11—Probes for Cytoskeletal Proteins
Chapter 12—Probes for Organelles
Chapter 13—Probes for Lipids and Membranes
Chapter 14—Fluorescent Tracers of Cell Morphology and Fluid Flow
Chapter 15—Assays for Cell Viability, Proliferation and Function
Chapter 16—Probes for Endocytosis, Receptors and Ion Channels
Chapter 17—Probes for Signal Transduction
Chapter 18—Probes for Reactive Oxygen Species, Including Nitric Oxide
Chapter 19—Indicators for Ca2+, Mg2+, Zn2+ and Other Metal Ions
Chapter 20—pH Indicators
Chapter 21—Indicators for Na+, K+, Cl– and Miscellaneous Ions
Chapter 22—Probes for Membrane Potential
Chapter 23—Antifades and Other Tools for Fluorescence Applications
List of Tables
Technical Notes and Product Highlights
Introduction to Enzyme Substrates and Their Reference Standards—Section 10.1
Substrates Yielding Soluble Fluorescent Products
Substrates for Live-Cell Enzyme Assays
Substrates Yielding Insoluble Fluorescent or Chromophoric Products
Substrates Based on Excited-State Energy Transfer
Fluorescent Derivatization Reagents for Discontinuous Enzyme Assays
Detecting Glycosidases—Section 10.2
Fluorogenic β-Galactosidase Substrates
Modified Fluorogenic β-Galactosidase Substrates with Improved Cellular Retention
NovaBright β-Galactosidase Chemiluminescent Detection Kits
Amplex Red Galactose/Galactose Oxidase Assay Kit
Fluorogenic β-Glucuronidase Substrates
Fluorogenic β-Glucosidase Substrates
Amplex Red Glucose/Glucose Oxidase Assay Kit
Amplex Red Neuraminidase/Sialidase Assay Kit
EnzChek Cellulase Substrate
EnzChek
Ultra
Amylase Assay Kit
EnzChek
Ultra
Xylanase Assay Kit
EnzChek Lysozyme Assay Kit
Chromogenic Glycosidase Substrates
Auxiliary Products for Glycosidase Research
Detecting Enzymes That Metabolize Phosphates and Polyphosphates—Section 10.3
Phosphatase Substrates Yielding Soluble Fluorescent Products
ELF 97 Phosphate: A Phosphatase Substrate That Yields a Fluorescent Precipitate
BODIPY FL ATP-γ-S and BODIPY FL GTP-γ-S Thioesters: Substrates for the Fhit Nucleotide-Binding Protein
Chemiluminescent Phosphatase Substrates
Chromogenic Phosphatase Substrate
Kits for Detecting Phosphatases, Polymerases and Nucleases
Antibody Beacon Tyrosine Kinase Assay Kit
ATP Determination Kit
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Chapter 10—Enzyme Substrates and Assays
(5.4 MB)
Detecting Peptidases and Proteases—Section 10.4
Peptidase Substrates
Caspase Substrates and Assay Kits
Substrates for HIV Protease and Renin
EnzChek Protease Assay Kits and Fluorescein Casein
EnzChek Gelatinase/Collagenase Assay Kit
EnzChek Elastase Assay Kit
DQ Substrates
Alternative Methods for Detecting Protease Activity
Protease Inhibitors
Substrates for Oxidases, Including Amplex Red Kits—Section 10.5
Peroxidases
Myeloperoxidase
Catalase
Glucose and Glucose Oxidase
Galactose and Galactose Oxidase
Cholesterol and Cholesterol Oxidase
Monoamine Oxidase
Glutamic Acid and Glutamate Oxidase
Acetylcholine, Acetylcholinesterase and Choline Oxidase
Xanthine and Xanthine Oxidase
Uric Acid and Uricase
Substrates for Microsomal Dealkylases, Acetyltransferases, Luciferases and Other Enzymes—Section 10.6
Microsomal Dealkylases
EnzChek Epoxide Hydrolase Substrate
Lipases
Chloramphenicol Acetyltransferase (CAT)
Luciferases
β-Lactamases
Dehydrogenases
Nitroreductase and Nitrate Reductase
Related Tables
Glycosidase enzymes and their fluorogenic and chromogenic substrates—Table 10.1
Rhodamine 110–based bis-peptide substrates—Table 10.2
RediPlate Assay Kits—Table 10.3
Detection limits of the EnzChek Protease Assay Kits—Table 10.4
Advantages of the Amplex UltraRed reagent over chromogenic reagents—Table 10.5