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Home
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Technical Reference Library
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The Molecular Probes Handbook
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Protein Detection and Analysis—Chapter 9
Protein Detection and Analysis—Chapter 9
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Chapter 9—Protein Detection and Analysis
Introduction to Protein Detection—Section 9.1
Protein Quantitation in Solution—Section 9.2
Protein Detection on Gels, Blots and Arrays—Section 9.3
Detecting Protein Modifications—Section 9.4
Peptide Analysis, Sequencing and Synthesis—Section 9.5
Molecular Probes Handbook
Table of Contents
Fluorescence Fundamentals
Chapter 1—Fluorophores and Their Amine-Reactive Derivatives
Chapter 2—Thiol-Reactive Probes
Chapter 3—Click Chemistry and other Functional Group Modifications
Chapter 4—Biotin and Hapten Derivatives
Chapter 5—Crosslinking and Photoactivatable Reagents
Chapter 6—Ultrasensitive Detection Technology
Chapter 7—Antibodies, Avidins and Lectins
Chapter 8—Nucleic Acid Detection and Analysis
Chapter 9—Protein Detection and Analysis
Chapter 10—Enzyme Substrates and Assays
Chapter 11—Probes for Cytoskeletal Proteins
Chapter 12—Probes for Organelles
Chapter 13—Probes for Lipids and Membranes
Chapter 14—Fluorescent Tracers of Cell Morphology and Fluid Flow
Chapter 15—Assays for Cell Viability, Proliferation and Function
Chapter 16—Probes for Endocytosis, Receptors and Ion Channels
Chapter 17—Probes for Signal Transduction
Chapter 18—Probes for Reactive Oxygen Species, Including Nitric Oxide
Chapter 19—Indicators for Ca2+, Mg2+, Zn2+ and Other Metal Ions
Chapter 20—pH Indicators
Chapter 21—Indicators for Na+, K+, Cl– and Miscellaneous Ions
Chapter 22—Probes for Membrane Potential
Chapter 23—Antifades and Other Tools for Fluorescence Applications
List of Tables
Technical Notes and Product Highlights
Introduction to Protein Detection—Section 9.1
Proteomics: A Rapidly Developing Field
Molecular Probes Detection Technology for Proteomics
Protein Quantitation in Solution—Section 9.2
Quant-iT and Qubit Protein Assay Kits
Qubit 2.0 Fluorometer
NanoOrange Protein Quantitation Kit
CBQCA Protein Quantitation Kit
EZQ Protein Quantitation Kit
Other Reagents for Protein Quantitation in Solution
Protein Detection on Gels, Blots and Arrays—Section 9.3
SYPRO Protein Gel Stains
Coomassie Fluor Orange Protein Gel Stain
Rhinohide Polyacrylamide Gel Strengthener
SYPRO Ruby Protein Blot Stain
Protein Detection on Microarrays
Protein Detection in Capillary Electrophoresis
Detecting Protein Modifications—Section 9.4
Click-iT Reagents for Detecting Protein Synthesis and Modifications
Pro-Q Diamond Phosphoprotein Stain for Gels and Blots
Pro-Q Glycoprotein Stain Kits for Gels and Blots
Other Specialized Techniques for Detecting Specific Proteins on Gels and Blots
Peptide Analysis, Sequencing and Synthesis—Section 9.5
N-Terminal Amino Acid Analysis
Peptide Sequencing
Peptide Synthesis
Get chapter downloads
Chapter 9—Protein Detection and Analysis
(2.4 MB)
Related Tables
A comparison of reagents for detecting and quantitating proteins in solution—Table 9.1
SYPRO and Coomassie Fluor luminescent and fluorescent protein gel stains—Table 9.2
Molecular Probes fluorescent and luminescent protein blot stains—Table 9.3
Pro-Q Diamond gel stain reagents and kits—Table 9.4
Pro-Q Emerald glycoprotein stain kits for gels and for blots—Table 9.5
Related Technical Notes
Monitoring Protein-Folding Processes with Environment-Sensitive Dyes—Note 9.1
Labeling Small Peptides with Amine-Reactive Dyes in Organic Solvents—Note 9.2