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Home
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Technical Reference Library
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The Molecular Probes Handbook
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Nucleic Acid Detection and Analysis—Chapter 8
Nucleic Acid Detection and Analysis—Chapter 8
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Chapter 8—Nucleic Acid Detection and Analysis
Nucleic Acid Stains—Section 8.1
Labeling Oligonucleotides and Nucleic Acids—Section 8.2
Nucleic Acid Quantitation in Solution—Section 8.3
Nucleic Acid Detection on Gels, Blots and Arrays—Section 8.4
Molecular Probes Handbook
Table of Contents
Fluorescence Fundamentals
Chapter 1—Fluorophores and Their Amine-Reactive Derivatives
Chapter 2—Thiol-Reactive Probes
Chapter 3—Click Chemistry and other Functional Group Modifications
Chapter 4—Biotin and Hapten Derivatives
Chapter 5—Crosslinking and Photoactivatable Reagents
Chapter 6—Ultrasensitive Detection Technology
Chapter 7—Antibodies, Avidins and Lectins
Chapter 8—Nucleic Acid Detection and Analysis
Chapter 9—Protein Detection and Analysis
Chapter 10—Enzyme Substrates and Assays
Chapter 11—Probes for Cytoskeletal Proteins
Chapter 12—Probes for Organelles
Chapter 13—Probes for Lipids and Membranes
Chapter 14—Fluorescent Tracers of Cell Morphology and Fluid Flow
Chapter 15—Assays for Cell Viability, Proliferation and Function
Chapter 16—Probes for Endocytosis, Receptors and Ion Channels
Chapter 17—Probes for Signal Transduction
Chapter 18—Probes for Reactive Oxygen Species, Including Nitric Oxide
Chapter 19—Indicators for Ca2+, Mg2+, Zn2+ and Other Metal Ions
Chapter 20—pH Indicators
Chapter 21—Indicators for Na+, K+, Cl– and Miscellaneous Ions
Chapter 22—Probes for Membrane Potential
Chapter 23—Antifades and Other Tools for Fluorescence Applications
List of Tables
Technical Notes and Product Highlights
Nucleic Acid Stains—Section 8.1
Properties of Cyanine Dyes
Premier Cyanine Dyes for Ultrasensitive Nucleic Acid Detection and Quantitation
Cell-Impermeant Cyanine Dimers: The TOTO Family of Dyes
Cell-Impermeant Cyanine Monomers: The TO-PRO Family of Dyes
Cell-Impermeant SYTOX Dyes for Dead-Cell Staining
Cell-Permeant Cyanine Dyes: The SYTO Nucleic Acid Stains
Amine-Reactive Cyanine Dye
Phenanthridines and Acridines: Classic Intercalating Dyes
Indoles and Imidazoles: Classic Minor Groove–Binding Dyes
Other Nucleic Acid Stains
Labeling Oligonucleotides and Nucleic Acids—Section 8.2
ChromaTide Nucleotides
Amine-Modified Nucleotides
ARES DNA Labeling Kits
FISH Tag DNA and FISH Tag RNA Kits
ULYSIS Nucleic Acid Labeling Kits
Labeled Oligonucleotides
Other Labeling Methods for Nucleic Acids
Nucleic Acid Quantitation in Solution—Section 8.3
Quant-iT and Qubit Assay Kits for DNA and RNA
Qubit 2.0 Fluorometer
PicoGreen dsDNA Quantitation Assay
OliGreen ssDNA Quantitation Assay
RiboGreen RNA Quantitation Assay
Other Stains for Nucleic Acid Quantitation in Solution
Real-Time Quantitative PCR Using SYBR Green I Nucleic Acid Gel Stain
Nucleic Acid Quantitation on Gels, Blots and Arrays—Section 8.4
Nucleic Acid Detection in Gels
Safe Imager Blue-Light Transilluminator
SYBR Photographic Filters
Capillary Electrophoresis and Channel Electrophoresis
Quality Control Testing on Microarrays and Blots
Related Tables
Specialty nucleic acid reagents for molecular biology—Table 8.1
Cell membrane–impermeant cyanine nucleic acid stains—Table 8.2
Cell-permeant cyanine nucleic acid stains—Table 8.3
Properties of classic nucleic acid stains—Table 8.4
Characteristics of Molecular Probes ChromaTide and aha labeled nucleotides—Table 8.5
Molecular Probes nucleic acid labeling kits—Table 8.6
Amine-reactive dyes for nucleic acid sequencing—Table 8.7
Selection guide for the Qubit and Quant-iT Assay Kits—Table 8.8
Related Technical Notes
SYBR Safe DNA Gel Stain—Note 8.1
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Chapter 8—Nucleic Acid Detection and Analysis
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