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Technical Reference Library
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The Molecular Probes Handbook
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Biotin and Hapten Derivatives—Chapter 4
Biotin and Hapten Derivatives—Chapter 4
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Chapter 4—Biotin and Hapten Derivatives
Introduction to Avidin–Biotin and Antibody–Hapten Techniques—Section 4.1
Biotinylation and Haptenylation Reagents—Section 4.2
Biotin and Desthiobiotin Conjugates—Section 4.3
Molecular Probes Handbook
Table of Contents
Fluorescence Fundamentals
Chapter 1—Fluorophores and Their Amine-Reactive Derivatives
Chapter 2—Thiol-Reactive Probes
Chapter 3—Click Chemistry and other Functional Group Modifications
Chapter 4—Biotin and Hapten Derivatives
Chapter 5—Crosslinking and Photoactivatable Reagents
Chapter 6—Ultrasensitive Detection Technology
Chapter 7—Antibodies, Avidins and Lectins
Chapter 8—Nucleic Acid Detection and Analysis
Chapter 9—Protein Detection and Analysis
Chapter 10—Enzyme Substrates and Assays
Chapter 11—Probes for Cytoskeletal Proteins
Chapter 12—Probes for Organelles
Chapter 13—Probes for Lipids and Membranes
Chapter 14—Fluorescent Tracers of Cell Morphology and Fluid Flow
Chapter 15—Assays for Cell Viability, Proliferation and Function
Chapter 16—Probes for Endocytosis, Receptors and Ion Channels
Chapter 17—Probes for Signal Transduction
Chapter 18—Probes for Reactive Oxygen Species, Including Nitric Oxide
Chapter 19—Indicators for Ca2+, Mg2+, Zn2+ and Other Metal Ions
Chapter 20—pH Indicators
Chapter 21—Indicators for Na+, K+, Cl– and Miscellaneous Ions
Chapter 22—Probes for Membrane Potential
Chapter 23—Antifades and Other Tools for Fluorescence Applications
List of Tables
Technical Notes and Product Highlights
Introduction to Avidin–Biotin and Antibody–Hapten Techniques—Section 4.1
Avidin–Biotin and Antibody–Hapten Techniques and Their Applications
Modified Avidin and Biotin
Biotinylation and Haptenylation Reagents—Section 4.2
Biotin and Biotinylation Reagents
Convenient Kits for Biotinylating Proteins
Biotin Quantitation Assay Kits
Haptenylation Reagents
Biotin and Desthiobiotin Conjugates—Section 4.3
Fluorescent Biotin Derivatives
Biotinylated Dextrans
Biotinylated and DSB-X Biotin–Labeled Proteins
Biotinylated Microspheres
Biotinylated Qdot Nanocrystals
Biotinylated Nucleotides
Biotinylated Site-Selective Probes
Biotinylated Lipids
Related Tables
Biotinylation and desthiobiotinylation reagents—Table 4.1
Molecular Probes avidin, streptavidin, NeutrAvidin and CaptAvidin conjugates—Table 7.9
Selected haptenylation reagents and their anti-hapten antibodies—Table 4.2
Sensitivity of the FluoReporter Biotin Quantitation Assay Kit for biotinylated nucleic acids with varying degrees of biotinylation—Table 4.3
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Chapter 4—Biotin and Hapten Derivatives
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