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The Molecular Probes Handbook
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Antibodies, Avidins and Lectins—Chapter 7
Antibodies, Avidins and Lectins—Chapter 7
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Chapter 7—Antibodies, Avidins and Lectins
Introduction to Antibodies, Avidins and Lectins—Section 7.1
Secondary Immunoreagents—Section 7.2
Zenon Technology: Versatile Reagents for Immunolabeling—Section 7.3
Anti-Dye and Anti-Hapten Antibodies—Section 7.4
Antibodies against Expression Tags—Section 7.5
Avidin and Streptavidin Conjugates—Section 7.6
Lectins and Other Carbohydrate-Binding Proteins—Section 7.7
Molecular Probes Handbook
Table of Contents
Fluorescence Fundamentals
Chapter 1—Fluorophores and Their Amine-Reactive Derivatives
Chapter 2—Thiol-Reactive Probes
Chapter 3—Click Chemistry and other Functional Group Modifications
Chapter 4—Biotin and Hapten Derivatives
Chapter 5—Crosslinking and Photoactivatable Reagents
Chapter 6—Ultrasensitive Detection Technology
Chapter 7—Antibodies, Avidins and Lectins
Chapter 8—Nucleic Acid Detection and Analysis
Chapter 9—Protein Detection and Analysis
Chapter 10—Enzyme Substrates and Assays
Chapter 11—Probes for Cytoskeletal Proteins
Chapter 12—Probes for Organelles
Chapter 13—Probes for Lipids and Membranes
Chapter 14—Fluorescent Tracers of Cell Morphology and Fluid Flow
Chapter 15—Assays for Cell Viability, Proliferation and Function
Chapter 16—Probes for Endocytosis, Receptors and Ion Channels
Chapter 17—Probes for Signal Transduction
Chapter 18—Probes for Reactive Oxygen Species, Including Nitric Oxide
Chapter 19—Indicators for Ca2+, Mg2+, Zn2+ and Other Metal Ions
Chapter 20—pH Indicators
Chapter 21—Indicators for Na+, K+, Cl– and Miscellaneous Ions
Chapter 22—Probes for Membrane Potential
Chapter 23—Antifades and Other Tools for Fluorescence Applications
List of Tables
Technical Notes and Product Highlights
Introduction to Antibodies, Avidins and Lectins—Section 7.1
Secondary Immunoreagents—Section 7.2
Species-Specific Secondary Antibodies
Image-iT FX Signal Enhancer
Alexa Fluor Signal Amplification Kits
Fluorescence- and Chemiluminescence-Based ELISA Kits
Fluorescence-Based Western Blot Kits
Gold-Labeled Immunoreagents
DAB Histochemistry Kits
Protein A and Protein G Conjugates
Zenon Technology: Versatile Reagents for Immunolabeling—Section 7.3
Features of Zenon Antibody Labeling Technology
Zenon Labeling Kits and Their Applications
Anti-Dye and Anti-Hapten Antibodies—Section 7.4
Anti-Dye and Anti-Hapten Antibodies
Efficient Quenching by Anti-Fluorophore Antibodies
Anti-Dinitrophenyl Antibody
Anti-Nitrotyrosine Antibody
Anti-Biotin Antibody
Antibodies against Expression Tags—Section 7.5
Antibodies Specific for Reporter Gene Products
Antibodies Specific for Epitope Tags
Avidin and Streptavidin Conjugates—Section 7.6
Binding Characteristics of Biotin-Binding Proteins
Fluorescent Avidin and Streptavidin Conjugates
Enzyme Conjugates of Avidin and Streptavidin
Gold-Labeled Streptavidin
Affinity Chromatography
Anti-Biotin Antibody: An Alternative to Avidin
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Chapter 7—Antibodies, Avidins and Lectins
(2.7 MB)
Lectins and Other Carbohydrate-Binding Proteins—Section 7.7
Concanavalin A and Wheat Germ Agglutinin and Their Conjugates
Other Carbohydrate-Binding Proteins and Their Conjugates
Related Tables
Summary of Molecular Probes secondary antibody conjugates—Table 7.1
Molecular Probes goat anti-mouse isotype-specific antibodies—Table 7.2
Alkaline phosphatase and horseradish peroxidase enzyme conjugates—Table 7.3
Fluorescent dyes successfully tested with the Image-iT FX signal enhancer—Table 7.4
NANOGOLD, Alexa Fluor FluoroNanogold and colloidal gold conjugates—Table 7.5
Binding profiles of protein A and protein G—Table 7.6
Zenon Antibody Labeling Kits—Table 7.7
Anti-fluorophore and anti-hapten antibodies—Table 7.8
Molecular Probes avidin, streptavidin, NeutrAvidin and CaptAvidin conjugates—Table 7.9
Molecular Probes lectin conjugates—Table 7.10
Related Technical Notes
Antibody Structure and Classification—Note 7.1
MHC Tetramer Technology—Note 7.2