Comparison with APC-H7. Mouse splenocytes stained with Anti-CD8 (clone 53-6.7) conjugated to APC-eFluor 780 (blue line) or APC-H7 (red line).
APC-eFluor 780 organic dye was developed to provide a high performance tandem fluorophore for multicolor flow cytometry. It was designed for use with flow cytometry instruments equipped with a red laser and has an emission peak of 780 nm. With similar spectral characteristics to APC-Alexa Fluor 750, APC-eFluor 780 requires no changes to your instrument configuration for use, allowing you to easily add APC-eFluor 780 to your multicolor panels.
Benefits of APC-eFluor 780 include
- Equal or better sensitivity than APC-H7 tandem conjugates
- Stable performance when treated with formaldehyde fixation or exposed to ambient light
- Uses same filter sets as existing comparative fluorochromes
APC-eFluor 780 dye dashboard
 Initial brightness | APC-eFluor 780 dye has similar spectral properties to APC-H7, APC-Cyanine7 and APC-Alexa Fluor 750 dye. It can replace any of these tandem dyes when designing a multicolor staining panel. The APC-eFluor 780 tandem dye is easily compensated when used with APC. | ||
 Photostability in buffer |
| 633 | 780/60 | 655 | 780 | ||
 Laser line |  Common filter set |  Excitation max |  Emission max |
Stability of APC-eFluor 780. Human peripheral blood mononuclear cells (PBMCs) were stained with Anti-CD8 APC-eFluor 780 (clone RPA-T8) and analyzed immediately (red line). The sample was then divided and either exposed to ambient light for six hours (blue line) or fixed in 2% formaldehyde for 30 minutes at room temperature (green line) and analyzed again.
APC-eFluor 780 dye products
We offer APC-eFluor 780 dye conjugated to primary antibodies for use in flow cytometry.
Learn more here:
Resources
5 steps resources
Related products
Brilliant Ultra Violet™ and Brilliant Violet™ are trademarks or registered trademarks of Becton, Dickinson and Company or its affiliates, and is used under license.
仅供科研使用，不可用于诊断目的。