Qdot 655 spectra excitation shown as dotted line and emission shown as solid red histogram
5350, 488, 595, 615655/20300654(in buffer) 5microscopy, flow cytometry

Invitrogen Qdot 655 probe is an excellent far-red–fluorescent label with outstanding brightness and photostability for imaging and flow cytometry applications. With an emission maximum of ~655 nm, Qdot 655 probe can be excited using 350, 488, 595, or 615 nm laser lines or a standard Cy5 filter. Optimum imaging results with brighter signals are achieved with a Qdot 655 filter or light cube.

Organic fluorophores and Qdot probes can be readily combined in multiplexed flow cytometry or imaging studies. Longer-wavelength Qdot probes are also well-suited for multiplexed western blots.

We offer Qdot 655 probe conjugated to a variety of antibodies, peptides, and proteins, optimized for cellular labeling and detection. In addition, reactive dye forms and antibody labeling kits are available to allow you to generate your own antibody conjugates or probes.

Qdot Label Conjugates for Cell and Tissue Staining    Qdot 655 primary antibodies

Experimental data using Qdot 655 probe
Flow scatter plot of Qdot 655-stained CD3 vs. Qdot 605-stained CD19

Flow cytometry analysis of hMNCs using streptavidin conjugates of Qdot 655 and Qdot 605. Human mononuclear cells (hMNCs) were sequentially stained with mouse anti-human CD3 (Biotin), Qdot 655 Streptavidin Conjugate, mouse anti-human CD19 (Biotin), and Qdot 605 Streptavidin Conjugate, with washes between each step. Lymphocytes were analyzed for fluorescence by flow cytometry using violet diode excitation, and detected using 605/20 nm and 655/20 nm emission filters. The dot plot shows CD3-positive cells (T cells) are mutually exclusive from CD19-positive cells (B cells).