NovaFluor Red 660 excitation shown as dotted line and emission shown as solid red histogram
4640660/20637658(in buffer) 5
flow cytometry

Invitrogen NovaFluor Red 660 dye has excitation and emission spectra similar to those of allophycocyanin (APC) and Alexa Fluor 647 dye. When excited by the red 640 nm laser line, the spectral spread of NovaFluor Red 660 dye is about half that of APC while maintaining similar brightness.

The macromolecule-based NovaFluor Red 660 dye produces highly stable fluorescence, and stained samples retain their fluorescence intensity and spectral signature when stored at 4°C. Use NovaFluor dyes with CellBlox Plus Blocking Buffer to reduce background and to block non-specific binding of NovaFluor labels, PE and APC tandems observed with macrophages and monocytes.

We offer NovaFluor dyes conjugated to primary antibodies for use in flow cytometry, as well as NovaFluor Antibody Conjugation Kits, NovaFluor CD4 Label Characterization Kits, and custom conjugation services.

Search NovaFluor Red 660 primary antibodies

Benefits of NovaFluor Red 660 dye
  • Narrow emission spectrum and minimal cross-laser excitation for better resolution
  • Bright fluorescence allows it to be paired with medium-to-low abundance antigens
  • Can serve as a replacement for APC with a cleaner emission spectrum, reducing spectral spillover for better marker resolution
  • Can be used in a flow cytometry panel with NovaFluor Red 685 or NovaFluor Red 700 dye
  • Produces highly stable fluorescence and is compatible with all buffers tested
Experimental data using NovaFluor Red 660 dye
Spectral signature of NovaFluor Red 660 dye. Normal human peripheral blood cells stained with CD4 Monoclonal Antibody (SK3 (SK-3)), NovaFluor Red 660 were used for analysis. Data was acquired on a 5-laser Cytek Aurora system.
: 2-panel scatter plots of human PBMCs, unstained vs. CD3e-stained and CD127-stained vs. CD3e-stained
Staining of human peripheral blood cells with NovaFluor Red 660 conjugated antibody. Normal human peripheral blood cells (PBMCs) were unstained (left) or stained with CD127 Monoclonal Antibody, NovaFluor Red 660 (right). All cells were co-stained with CD3e Monoclonal Antibody, eFluor 450. Total viable cells in the lymphocyte gate were used for analysis, as determined by LIVE/DEAD Fixable Blue Dead Cell Stain Kit, for UV excitation. Data was acquired on a 5-laser Cytek Aurora and unmixed with autofluorescence extraction.