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1 | 405 | 510/50 | 419 | 508 | (in buffer) 1 | flow cytometry |
The eFluor 506 organic dye is a violet-laser excitable fluorophore with an emission peak of 506 nm and is optimized for use in flow cytometry applications. With similar spectra characteristics to AmCyan and Horizon V500 dyes, conjugates of eFluor 506 can be easily added to your multicolor panels without change in instrument configuration for use.
We offer eFluor 506 dye conjugated to primary antibodies for use in flow cytometry.
Comparison of fluorescence intensity of eFluor 506 dye with Horizon V500. Mouse splenocytes were stained with CD4 Monoclonal Antibody (RM4-5), eFluor 506 (purple line) or Horizon V500 (blue histogram), using the same concentration of antibody.
Stability of eFluor 506 dye. Mouse splenocytes were stained with CD4 Monoclonal Antibody (RM4-5), eFluor 506 and either left unfixed (blue line), or were fixed for 30 minutes in Foxp3/Transcription Factor Buffer Set (purple line) or 30 minutes in IC Fixation Buffer followed by methanol (orange line).